菹草乙酸乙酯组分抑藻活性物质的分离纯化和鉴定

前期研究表明,菹草乙酸乙酯组分能显著抑制赤潮微藻米氏凯伦藻的生长.在此基础上,采用硅胶柱层析和硅胶GF254薄层层析等方法,分离纯化菹草乙酸乙酯组分中的抑藻活性物质.进一步应用质谱、核磁共振碳谱和氢谱等技术,鉴定抑藻活性物质.结果表明,从菹草乙酸乙酯组分中分离纯化到2种抑藻活性物质.当浓度为16μg·m L-1时,它们对米氏凯伦藻表现出一定的抑藻活性.结构鉴定表明,此2种抑藻活性物质为Trichodermatides B和2-methylheptylisonicotinate.此2种化合物为首次从菹草中分离得到,也是首次从沉水植物中分离得到.

Isolation, Purification and Identification of Antialgal Activity Substances of Ethyl Acetate Extracts from the Submerged MacrophytesPotamogeton crispus

Previous studies showed that ethyl acetate extracts from the submerged macrophytes Potamogeton crispus can significantly inhibit the growth of Karenia mikimitoi.Further, two antialgal activity compounds (1-2) were successfully isolated from this submerged macrophytes through a combination of silica gel column chromagraphy and repeated preparative thin-layer chromatography in this paper.These two antialgal activity compounds exhibited antialgal active against Karenia mikimitoi.Furthermore, their structure were identified on the basis of spectroscoic data: one flavonid named Trichodermatides B, and one alkaloid named 2-methylheptylisonicotinate.These two compounds were for the first time isolated from both Potamogeton crispus and submerged macrophytes.