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Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb^3+-tiron complex as a fluorescence probe
引用本文:LV Xue-fei,ZHAO Yi-bing,ZHOU Qun-fang,JIANG Gui-bin,SONG Mao-yong. Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb^3+-tiron complex as a fluorescence probe[J]. 环境科学学报(英文版), 2007, 19(5): 616-621. DOI: 10.1016/S1001-0742(07)60102-8
作者姓名:LV Xue-fei  ZHAO Yi-bing  ZHOU Qun-fang  JIANG Gui-bin  SONG Mao-yong
作者单位:[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beifing 100085, China [2]Department of Chemistry, the Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China
基金项目:国家自然科学基金 , 国家重点基础研究发展计划(973计划)
摘    要:A sensitive method based on the fluorescence quenching effect of the Tb^3+-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N=2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.

关 键 词:鱼浆 不耐碱磷蛋白 磷 测定 铽^3+ 钛试剂 荧光探针 指示生物
收稿时间:2006-05-22
修稿时间:2006-08-08

Determination of alkali-labile phosphoprotein phosphorus from fish plasmausing the Tb3+-tiron complex as a fluorescence probe
LV Xue-fei,ZHAO Yi-bing,ZHOU Qun-fang,JIANG Gui-bin and SONG Mao-yong. Determination of alkali-labile phosphoprotein phosphorus from fish plasmausing the Tb3+-tiron complex as a fluorescence probe[J]. Journal of environmental sciences (China), 2007, 19(5): 616-621. DOI: 10.1016/S1001-0742(07)60102-8
Authors:LV Xue-fei  ZHAO Yi-bing  ZHOU Qun-fang  JIANG Gui-bin  SONG Mao-yong
Affiliation:1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
2. Department of Chemistry, the Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China
Abstract:A sensitive method based on the fluorescence quenching effect of the Tb3 -Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N=2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.
Keywords:alkali-labile phosphoprotein phosphorus (ALP)  Chinese loach  fluorescence quenching  Tb3 -Tiron complex  vitellogenin  plasma  fish  phosphorus  Determination  fluorescence probe  complex  high sensitivity  lack  organic solvents  present  simple  procedure  results  good  colorimetric assay  relative  standard deviation  fluorescence quenching  detection  Tiron
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