光生物反应器培养紫球藻及藻粉降血脂的研究

研究了搅拌式光生物反应器中搅拌速度、通气量对紫球藻生长、胞外多糖和藻细胞超微结构的影响．实验结果表明：在特定光照(1000μmolm^-2s^-1)和培养温度(25℃)条件下，当搅拌转速为200r／min、通气量为0．71vvm时，藻生长速度和胞外多糖含量最高；增加通气量有利于藻细胞胞外多糖及生物量的提高．对藻细胞超微结构的观察发现，与摇床培养相比，搅拌式光生物反应器使藻细胞淀粉颗粒明显增多，粘质鞘层变薄，光合片层增多．动物实验表明，紫球藻能明显降低摄食高脂饲料SD大鼠血脂TC、TG、LDL-C水平，并提高HDL-C含量．实验结果预示，紫球藻对预防动脉粥样硬化和冠心病的发生有一定的意义．图2表3参26

CULTURE OF PORPHYRIDIUM CRUENTUM IN PHOTOBIOREACTOR AND ITS EFFECT ON HYPOLIPIDEMIAC

This study was aimed at developing mass culture of Porphyridium creutum in a 5.0 L stirred photobioreactor. Effect of agitation and aeration rate on cell growth, extracellular polysaccharide product and cell ultrastructure were studied. Under the experimental photon flux density ( 1 000 μmol m-2s -1 ) and temperature (25 ℃ ) , the high growth and product of extracellular polysaccharide was observed at the agitation 200 r/min and aeration rate 0.71 vvm, and high aeration rate made biomass production and extracellular polysaccharide content increased. Comparing with the reciprocating shaker, the cells grown in the stirred photobioreactor contained greater number of starch grains, cytoplasmic vacuoles, chloroplast lamellae and thiner delatinous sheaths. The level of TC, TG and LDL-C in the rats fed with the diet containing P. cruentum biomass was significantly lower and the HDL-C level significantly higher than those in the high lipid control group. These results indicated that algae might be significant to control atherosclerosis and coronary heart disease. Fig 2, Tab 3, Ref 26