沼泽红假单胞菌W12对活性黑5的厌氧脱色和降解作用

从处理印染废水的厌氧移动床生物膜反应器（moving bed biofilm reactor, MBBR）中分离到一株具有高效脱色活性的沼泽红假单胞菌W12。经实验确定W12对活性黑5（reactive black 5，RB5）脱色的适宜条件为：pH＜10；有光照；谷氨酸盐或乳酸盐作为碳源，当乳酸钠为碳源时浓度应＞500 mg/L；盐度不超过5％；RB5浓度不大于700 mg/L。紫外可见光谱扫描结果表明，RB5的脱色和降解过程生成芳香胺类化合物，这些中间产物可进一步降解。此外发现，RB5诱导生成的胞外代谢物能提高W12的脱色活性。

Anaerobic decolorization and degradation of reactive black 5 by Rhodopseudomonas palustris W12

A strain of photosynthetic bacterium, Rhodopseudomonas palustris W12, isolated from a lab scale anaerobic moving bed biofilm reactor (MBBR) treating textile effluent was demonstrated to decolorize reactive black 5 (RB5) efficiently. By a series of batch tests, the suitable conditions for RB5 decolorization were obtained, namely pH < 10, light presence, glutamine or lactate as carbon source with concentration more than 500 mg/L when lactate is selected, NaCl concentration not exceeding 5%, and RB5 concentration less than 700 mg/L. UV-vis spectrum scan was conducted to analyze the metabolic products of RB5 decolorization by W12. The results showed that aromatic amines produced with RB5 reduction were further degraded during the extended period. In addition, extracellular metabolites produced from RB5 inducement are proved to stimulate the biodecolorization by W12.