地下水石油污染高效生物降解研究

考察了高效复合微生物对地下水石油污染物降解效果,并建立了地下水污染质生物降解迁移数学模型.从石油污染的土壤中分离筛选到能够高效降解石油的菌株,经鉴定为假单胞菌属、黄杆菌属和微球菌属,这3种菌属24h对石油降解率分别为62%,56%和52%,且3种菌属组成的复合菌较单一菌属对石油降解率都要高,达85%.高效复合菌与石油配水一起进入模拟地下含水介质的反应器,在反应器前部均能形成一个稳定的生物带.石油配水流经该生物带,石油降解率可达60%以上,反应器出水石油降解率平均可达90%以上.建立的地下水污染质生物降解迁移数学模型对地下水有机污染质生物降解有较好的预测效果,计算值与实测值呈良好的相关性.

Study on the Groundwater Petroleum Contaminant Biodegradation by High Efficient Microorganism

The groundwater petroleum contaminant biodegradation effect by high efficient hybrid microorganism was investigated and the groundwater contaminant biodegradation transportation mathematical model was established in this study.The high efficient bacteria was separated and filtrated from the soil contaminated by petroleum hydrocarbon,and it was identified as Pseudomonas,Flavobacterium and Micrococcus.The petroleum degradation rates by these three kinds microorganism were 62%,56% and 62% respectively in 24 h,and the high petroleum biodegradation rate 85% could be achieved by the hybrid microorganism constituted by these three kinds of microorganism,which was higher than that of any other each single bacteria community.The hybrid microorganism in-flowed into the reactor imitating the aquifer media with the petroleum wastewater,and it could form a steady microorganism zone in the foreside of the reactor.The petroleum biodegradation rate could achieve 60% when the petroleum wastewater flowed through this microorganism zone and the average petroleum biodegradation rate could reach up to 90% in the effluent.The groundwater contaminant biodegradation transportation mathematical model can predict the biodegradation of the wastewater through the microorganism zone effectively,of which the calculating values have good relativity with those of measurement.