| 氯菊酯农药抗体制备及酶标抗原合成 |
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| 引用本文： | 刘廷凤,刘亚子,孙成.氯菊酯农药抗体制备及酶标抗原合成[J].中国环境科学,2005,25(5):0-0. |
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| 作者姓名： | 刘廷凤 刘亚子 孙成 |
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| 作者单位： | 南京大学环境学院,污染控制与资源化国家重点实验室 江苏南京210093 |
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| 基金项目： | 国家自然科学基金资助项目(29877010);江苏省自然科学基金资助项目(BK2002088) |
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| 摘 要： | 为建立一种快速检测氯菊酯的酶免疫学方法,以自制的氯菊酯半抗原(Py)与牛血清白蛋白(BSA)偶联得到免疫原.免疫健康新西兰白兔获得特异性多克隆抗体.用间接酶联免疫吸附法(ELISA)鉴定抗体效价为1:2500和1:4000.合成了辣根过氧化物酶(HRP)标记抗原(HRP-Py),并经紫外分光光度法鉴定,摩尔分子比为2.5:1.
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| 关 键 词： | 氯菊酯 抗体 酶标抗原 |
| 文章编号： | 1000-6923(2005)05-0615-03 |
| 收稿时间： | 1900-01-01; |
| 修稿时间： | 2004年12月7日 |
Preparation of pesticide antibody and enzyme labeled antigen synthesis for permethrin |
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| LIU Ting-feng,LIU Ya-zi,SUN Cheng.Preparation of pesticide antibody and enzyme labeled antigen synthesis for permethrin[J].China Environmental Science,2005,25(5):0-0. |
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| Authors: | LIU Ting-feng LIU Ya-zi SUN Cheng |
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| Institution: | LIU Ting-feng,LIU Ya-zi,SUN Cheng* |
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| Abstract: | To establish a kind of competitive ELISA technique for rapid detecting of permethrin, residue Bovine serum albumin (BSA) was used to couple with self-made semi-antigen permethrin (Py) to obtain immunity source. The complete-antigen BSA-Py acted as immunoantigen to obtain the specific polyclonal antibody. The effect values of antibody were identified through indirect competitive ELISA to be 1:2500 and 1:4000. The HRP labeled antigen (HRP-Py) was synthesized and identified with UV and the molar ratio was 2.5:1. |
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| Keywords: | permethrin antibody enzyme labeled antigen |
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