染色体组荧光原位杂交及C—带鉴定小麦中的簇毛麦染色体

通过分子细胞生物学方法，对抗白粉病、高蛋白质含量“小麦×簇毛麦”杂种后代端体附加系进行染色体组荧光原位杂交，以鉴定附加染色体来源．结果表明，附加端体染色体为簇毛麦染色体，染色体C－带分析结果显示，该端体可能为簇毛麦6Vs或7Vs染色体．该端体染色体携有抗白粉病和高蛋白质基因，利用染色体显微操作技术可对这些优良基因进行分离、克隆并加以利用．染色体原位杂交结果还发现，通过“小麦－簇毛麦”易位系6Vs／7AL易位染色体转移操作，已将易位染色体转移到推广小麦品种“川育12”中．此外，还观察到同源染色体在细胞内为相邻排列，而不是随机分布于核内

GENOMIC IN SITU HYBRIDIZATION AND C-BANDING APPROACHES FOR IDENTIFYING HAYNALDIA VILLOSA CHROMOSOME IN WHEAT LINES

Detection of the Haynaldia chromosomes in telosomic addition and translocation lines of commonwheat, Triticum acstivum L., was undertaken using genomic fluorescent in situ hybridization(GFISH) andCbanding techniques. The results indicated that the added chromosome originated from H. villosa. Analysis ofCbanding showed the alien telosomic chromosome was probably 6Vs or 7Vs of H. villosa. This chromosome carriedthe powerdery mildew resistant gene and high protein content genes, which could be isolated, cloned and utilizedusing micromanipulation technique The result from GFISH revealed that the translocated chromosome 6VS/6ALhad been transferred into the cultivated variety"Chuanyu 12". Simultaneously, it was observed that thehomologous chromosomes arranged adjacently and not randomly in cells.