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Salinity and nutrient effects on the induction of the galactose permease system in a psychrophilic marine vibrio
Authors:S S Hayasaka  R Y Morita
Institution:(1) Department of Microbiology, Oregon State University, Corvallis, Oregon, USA;(2) School of Oceanography, Oregon State University, Corvallis, Oregon, USA;(3) Department of Microbiology and School of Oceanography, Oregon State University, 97331 Corvallis, Oregon, USA;(4) Department of Microbiology, Clemson University, 29631 Clemson, South Carolina, USA
Abstract:An Antarctic psychrophilic marine Vibrio species was isolated having the inducible ability to accumulate nonmetabolizable thiomethyl-14C-beta-galactopyranoside (14C-TMG) through a galactose permease system. Induction of 14C-TMG uptake was found to have a salinity requirement which was higher than that required for uptake. At the optimum salinity, galactose and fucose were the primary inducers. Lactose produced a comparable induction but only at higher concentrations, whereas glucose did not cause induction. The initial rate of 14C-TMG uptake exhibited saturation kinetics with an apparent Km value of 4.8 x 10–6M. An amino acid, in addition to the inducer, was required for induction which could not be replaced by glycerol or galactose. Evidence is presented which indicates that the uptake of 14C-TMG is energy-dependent and that nutrient availability is more important than salinity for induction and uptake under conditions which would normally be found in the oceanic environment.Technical Paper No. 4911, Oregon Agricultural Experiment Station.
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