Overexpression of NADH oxidase gene from Deinococcus geothermalis in Escherichia coli |
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| Authors: | Sase Kazuya Iwasaki Tomomi Karasaki Hatsune Ishikawa Masahide |
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| Institution: | 1. Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan;2. Department of Life Science and Green Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan;1. Department of Forest Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea;2. Department of BIN Fusion Technology, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 561-756, Republic of Korea;3. Department of Biotechnology, Vels University, Chennai 600117, India;1. State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China;2. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434023, PR China;3. Zhongnan Hospital, Wuhan University, Wuhan 430071, PR China;1. School of Environmental Science and Technology, Fujian Normal University, Fuzhou 350007, Fujian Province, China;2. Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, SA 5095, Australia;3. Cooperative Research Centre for Contamination Assessment and Remediation of Environments, Mawson Lakes, SA 5095, Australia;1. School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, People’s Republic of China;2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, People’s Republic of China |
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| Abstract: | When using stable enzyme genes from a thermophile to create a biosensor in Escherichia coli, it is vital that these genes be overexpressed in order to provide a sufficient supply of enzymes. In this study, overexpression of the NADH oxidase (Nox) gene from the thermophile Deinococcus geothermalis was successfully achieved with the aim of creating a stable biosensor active at room temperatures. To do so, modification of 10 nucleotides, GAAATTAACT, upstream of the start codon of the Nox gene was necessary. |
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| Keywords: | biosensor stable enzyme overexpression |
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