| 尿中1-羟基芘的同步荧光测定法 |
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| 引用本文： | 赵振华,田德海,全文熠.尿中1-羟基芘的同步荧光测定法[J].环境化学,1994,13(2):141-145. |
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| 作者姓名： | 赵振华 田德海 全文熠 |
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| 作者单位： | 北京市环境保护科学研究所!北京100037 |
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| 基金项目： | 北京市自然科学基金资助项目 |
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| 摘 要： | 本文报告1-羟基芘的荧光光谱在碱性溶液中的红移现象,研究了在1mol/l NaOH溶液中的同步荧光光谱,得出最适△λ为35nm.以此为基础建立了尿中1-羟基芘的同步荧光测定法,回收率80%,精密度8.0%,检测下限0.05ng/ml.
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| 关 键 词： | 同步荧光 1-羟基芘 红移现象 |
DETERMINATION OF URINARY 1-HYDROXYPYRENE WITH SYNCHRONOUS FLUORESCENCE TECHNIQUE |
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| Zhao Zhenhua Tian Dehai Quan Wenyi.DETERMINATION OF URINARY 1-HYDROXYPYRENE WITH SYNCHRONOUS FLUORESCENCE TECHNIQUE[J].Environmental Chemistry,1994,13(2):141-145. |
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| Authors: | Zhao Zhenhua Tian Dehai Quan Wenyi |
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| Abstract: | The red shift of fluorescence spectra for 1-hydroxypyrcne in lmol/1 NaOH solution is observed as the dielectric constant of the solvent increases. The synchronous fluorescence spectra of 1-hydroxypyrene in NaOH solution was studied. It was shown tnat the optimum difference AX of wavelength between excitation and emission for 1-hydroxypyrene in lmol/1 NaOH solution is 35nm, and the peak of spectrum is at 440nm.A sensitive method for urinary 1-hydroxypyrene with synchronous fluorescence spectroscopy was developed. The minimum detection limit of 1-hydroxypyrene is 0.05 ng and the linear range is over four order of magnitude, the recovery was observed over 80%. The results obtained with this method were comparable with those obtained with high performance liquid chromatography. |
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| Keywords: | synchronous fluorimetry red shift 1-hydroxypyrene |
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