| 一株降解邻苯二甲酸酯真菌的筛选及其降解特性研究 |
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| 引用本文： | 韩蕊,王冬莹,芮洋,李诗殷,吴文成,蔡信德. 一株降解邻苯二甲酸酯真菌的筛选及其降解特性研究[J]. 环境科学学报, 2013, 33(11): 2941-2946 |
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| 作者姓名： | 韩蕊 王冬莹 芮洋 李诗殷 吴文成 蔡信德 |
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| 作者单位： | 环境保护部华南环境科学研究所, 广州 510655;1. 环境保护部华南环境科学研究所, 广州 510655;2. 中国环境科学研究院, 北京 100012;环境保护部华南环境科学研究所, 广州 510655;环境保护部华南环境科学研究所, 广州 510655;环境保护部华南环境科学研究所, 广州 510655;环境保护部华南环境科学研究所, 广州 510655 |
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| 基金项目： | 国家环保公益科研专项(No.201109020) |
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| 摘 要： | 采用富集培养法,从PAEs污染的农田土壤中筛选出1株邻苯二甲酸酯类化合物(PAEs)降解真菌F9,经形态学特征及18S rDNA序列分析,初步鉴定为爪哇正青霉(Eupenicillum javanicun).通过正交试验研究,得出菌株F9的最优降解条件是:C:N为20:1、pH为7.0、最佳PAEs初始浓度为50 mg·L-1.菌株F9对土壤中复合PAEs(DMP、DEP和DOP)有良好的降解效果.在30 d培养期内,可将灭菌土壤中300 mg·kg-1的PAEs降解65.2%,且培养第一阶段(0~15 d)的降解率远高于第二阶段(16~30 d).
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| 关 键 词： | 邻苯二甲酸酯 真菌 土壤污染 生物降解 |
| 收稿时间： | 2013-06-16 |
| 修稿时间： | 2013-07-24 |
Biodegradation of PAEs by a newly isolated fungus Eupenicillum javanicun F9. |
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| HAN Rui,WANG Dongying,RUI Yang,LI Shiyin,WU Wencheng and CAI Xinde. Biodegradation of PAEs by a newly isolated fungus Eupenicillum javanicun F9.[J]. Acta Scientiae Circumstantiae, 2013, 33(11): 2941-2946 |
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| Authors: | HAN Rui WANG Dongying RUI Yang LI Shiyin WU Wencheng CAI Xinde |
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| Affiliation: | South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655;1. South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655;2. Chinese Research Academy of Environmental Sciences, Beijing 100012;South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655;South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655;South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655;South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655 |
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| Abstract: | A fungus F9 which could degrade phthalate esters (PAEs) was isolated from PAEs-contaminated agriculture soil. This strain of F9 was identified as Eupenicillum javanicun based on the morphological characteristics and 18S rDNA sequence analysis. The optimized PAEs degradation condition was reached by orthogonal design, with C:N=20:1, pH=7 and 50 mg·L-1 of original PAEs concentration. Eupenicillum javanicun had favorable effect on multi-PAEs (DMP, DEP and DOP) degradation in soil. Results showed that 65.2% of 300 mg·kg-1 PAEs soil was degraded during 30 d incubation period, with the first stage (0~15 d) far higher than the second stage (16~30 d). |
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| Keywords: | PAEs fungus soil contamination biodegradation |
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