| 马粪海胆RAPD反应体系的优化 |
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| 引用本文： | 刚锰,杨柏林,姜玲玲,王玉,熊德琪.马粪海胆RAPD反应体系的优化[J].海洋环境科学,2012(5):674-676. |
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| 作者姓名： | 刚锰 杨柏林 姜玲玲 王玉 熊德琪 |
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| 作者单位： | 大连海事大学环境科学与工程学院 |
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| 基金项目： | 国家自然科学基金(40976064);中央高校基本科研业务费专项资金(2011QN143) |
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| 摘 要： | 以马粪海胆为研究对象,从影响RAPD实验结果的4个因素(引物、dNTP、Taq酶、DNA)入手,进行单因素设计,从而寻找最佳的反应浓度,达到最佳的反应效果。优化后4种因素的最佳反应浓度分别为:引物100μmol/L、dNTP 2 500μmol/L、Taq酶2U、DNA稀释80倍。在优化的反应条件下,得到的产物重复性和稳定性都比较不错。
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| 关 键 词： | RAPD 单因素设计 马粪海胆 |
Optimization of RAPD reaction system of Hemicentrotus pulcherrimus |
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| GANG Meng,YANG Bo-lin,JIANG Ling-ling,WANG Yu,XIONG De-qi.Optimization of RAPD reaction system of Hemicentrotus pulcherrimus[J].Marine Environmental Science,2012(5):674-676. |
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| Authors: | GANG Meng YANG Bo-lin JIANG Ling-ling WANG Yu XIONG De-qi |
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| Institution: | (College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China) |
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| Abstract: | In the experiment,the stable and reliable experiment results of Hemicentrotus pulcherrimus were obtained.Four major factors(primer,dNTP,Taq enzyme and DNA) that greatly affected the results of RAPD were chosen and carried out single factor design to seek optimal reaction concentration.After the optimization concentrations of single factor design,we find out that 100 μmol/L of primer,2 500 μmol/L of dNTP,2 U of Taq enzyme,Diluted 80-fold of DNA.Under the optimized reaction conditions,the results of RAPD were both good in stability and repeatability. |
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| Keywords: | RAPD single factor design Hemicentrotus pulcherrimus |
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