Chorionic villus sampling: Improved method for preparation of karyotypes after short-term incubation |
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| Authors: | G L Terzoli F Lalatta F Gilbert |
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| Institution: | 1. Laboratorio di Citogenetica Instituti Clinici di Perfezionamento, Via della Commenda 12, 20122, Milano, Italy;2. Division of Medical Genetics, Mount Sinai School of Medicine, Fifth Avenue and 100th Street, New York, NY 10029, U.S.A |
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| Abstract: | A method for the isolation and trypsin-Giemsa banding of metaphases obtained after short-term incubation (48 h) of cytotrophoblast cells from chorionic villus sample is described. A new slide-making instrument, developed expressly to enhance the spreading of chromosomes from metaphases released from small tissue pieces, is responsible for the increase yield of analysable metaphases in this protocol. |
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