A simple and reliable method of chromosome banding for prenatal cytogenetics using a bromodeoxyuridine pulse |
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| Authors: | S Z Eichenbaum E J Krumins |
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| Institution: | Cytogenetics Laboratory, Department of Pathology, The Royal Women's Hospital, 132 Grattan Street, Carlton, 3053, Victoria, Australia |
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| Abstract: | An extremely simple, fully defined and reliable technique for banding chromosomes in situ is described. The method uses a pulse of bromodeoxyuridine to produce replication pattern banding complete with G- and C-type banding in the same karyotype. This enables immediate resolution of problems otherwise requiring extra subculturing and the application of conventional banding techniques. The value of routine chromosome banding in prenatal cytogenetics using such an economical technique is discussed. |
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| Keywords: | Prenatal cytogenetics In situ banding Bromodeoxyuridine pulse labelling |
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