A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures |
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| Authors: | Sau W Cheung James P Crane Ann Johnson Lyn Simms Joan Reid |
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| Institution: | Genetics Division, Department of Obstetrics and Gynecology, Washington University School of Medicine, Jewish Hospital of St. Louis, 216 South Kingshighway, St. Louis, Missouri 63110, U.S.A |
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| Abstract: | A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin-EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up ot 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations. |
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| Keywords: | Amniotic fluid cell culture Prometaphase chromosomes Prenatal diagnosis |
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