Microculture of fetal blood for prenatal cytogenetic studies from blood-stained amniotic fluid |
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Authors: | F Dagna Bricarelli A Arslanian L Doria Lamba Carbone R Chisci M Gessaga P Zucchinetti |
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Institution: | 1. Chair of Human Genetics, University of Genoa-Cytogenetic and Prenatal Diagnosis Laboratory, Galliera Hospital, Genoa, Italy;2. Department of Pediatrics, Galliera Hospital, Genoa, Italy |
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Abstract: | An alternative method to the culture of amniotic fluid cells for prenatal diagnosis of chromosome disorders is proposed. Microculture of fetal blood can be used when fetal blood is drawn at amniocentesis through accidental puncture of the placenta. An easy discrimination of fetal red cells, a good response of fetal lymphocytes to PHA and the possibility of identification of the fetal karyotype from the maternal one are the technical bases of this method. This technique offers some undoubted advantages: a reduced need for repeating amniocentesis because of a lack of growth of AF cells due to massive contamination with red cells; a result may be obtained sooner. Thirty-seven cases out of 1092 amniocenteses were processed in this way (3·4 per cent). In two cases no mitoses were obtained but in the others the diagnosis was confirmed by the results of AF cell culture and/or by the outcome of pregnancy. |
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Keywords: | Prenatal diagnosis Placentacentesis Lymphocyte microculture Amniotic fluid cell culture |
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