Amniotic fluid protease activity and the prenatal detection of cystic fibrosis |
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Authors: | L Poenaru M C Vinet |
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Institution: | Institute de Pathologic et Biologie Cellulaire et Moleculaire, INSERM U129, 24 rue du Fg Saint Jacques, 75674 Paris Cedex 14, France |
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Abstract: | Changes in the protease activity in amniotic fluid has been proposed as a valid method for the prenatal detection of cystic fibrosis (CF). We have studied by quantitative and qualitative procedures, sixty four amniotic fluids: two of them from CF-affected fetuses. Interpretation of the benzoyl arginine ethyl ester (BAEE)-staining patterns after isoelectric focusing was often difficult, and repeated experiments gave variable results. In order to improve gel discrimination, we performed amniotic fluid electrofocusing in the presence of detergents: 0.1 per cent Triton X-100, 0.1 per cent DOC, or 0.1 per cent SDS. In these conditions, the pattern revealed by BAEE was modified, but no differences were observed between CF and normal amniotic fluids. |
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Keywords: | Amniotic fluid arginine esterase Isoelectric focusing Cystic fibrosis Prenatal diagnosis |
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