| 鼠Tcp11l1基因的结构与表达分析 |
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| 引用本文： | 刘燕燕,王新颖,孙华钦,卢亦路,曾梅,陶大昌,刘运强,马用信. 鼠Tcp11l1基因的结构与表达分析[J]. 应用与环境生物学报, 2008, 14(6). DOI: 10.3724/SP.J.1145.2008.00783 |
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| 作者姓名： | 刘燕燕 王新颖 孙华钦 卢亦路 曾梅 陶大昌 刘运强 马用信 |
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| 作者单位： | 四川大学华西医院医学遗传研究室,生物治疗国家重点实验室疾病基因组学研究室,成都,610041 |
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| 基金项目： | 国家自然科学基金 ， "863"计划项目(No.2007AA02Z127)资助 Supported by the National Natural Science Foundation of China ， the State "863"Program of China |
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| 摘 要： | 为了分析鼠Tcp11l1基因的结构和表达,从小鼠睾丸组织总cDNA中扩增鼠Tcp11l1基因的开读框架(Open reading frame,ORF),定向克隆到真核表达载体pEGFP-N1,构建融合表达质粒pTcp11l1-EGFP,转染HEK293细胞,荧光显微镜下观察Tcp11l1基因的亚细胞定位;设计跨小鼠Tcp11l1基因两个外显子的引物,RT-PCR分析此基因在小鼠各组织中的表达情况.并利用生物信息学的方法对鼠Tcp11l1基因的结构进行初步预测.转染pTcp11l1-EGFP后在胞质中能明显看到绿色荧光信号,而在胞核和胞膜中无绿色荧光信号,表明鼠Tcp11l1蛋白定位于细胞质;RT-PCR分析结果表明,鼠Tcp11l1基因在小鼠各组织中广泛表达.生物信息学结果表明,鼠Tcp11l1和鼠Tcp11的蛋白具有相同的TCP11结构域,不能确定是否存在跨膜序列.鼠Tcp11l1基因和鼠Tcp11基因具有相似的亚细胞定位和TCP11结构域,表明这两个基因可能具有相似的受体功能.但是与Tcp11特异表达于睾丸组织的延长精细胞和精子不同,Tcp11l1为广泛表达,说明Tcp11l1可能在多种组织细胞中发挥作用.
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| 关 键 词： | Tcp11l1摹因 亚细胞定位 表达分析 跨膜分析 |
Expression and Structure Analysis of a Novel Mouse Gene Tcp11l1 |
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| LIU Yanyan,WANG Xinying,SUN Huaqin,LU Yilu,ZENG Mei,TAO Dachang,LIU Yunqiang,MA Yongxin. Expression and Structure Analysis of a Novel Mouse Gene Tcp11l1[J]. Chinese Journal of Applied and Environmental Biology, 2008, 14(6). DOI: 10.3724/SP.J.1145.2008.00783 |
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| Authors: | LIU Yanyan WANG Xinying SUN Huaqin LU Yilu ZENG Mei TAO Dachang LIU Yunqiang MA Yongxin |
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| Affiliation: | LIU Yanyan,WANG Xinying,SUN Huaqin,LU Yilu,ZENG Mei,TAO Dachang,LIU Yunqiang & MA Yongxin (Division of Human Morbids Genomics of State Key Laboratory of Biotherapy,Department of Medical Genetics,West China Hospital,Sichuan University,Chengdu 610041,China) |
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| Abstract: | To investigate the structure and expression pattern of mouse Tcp11l1 (mTcp11l1) gene, the pEGFP-mTcp11l1 plasmid encoding pEGFP-mTcp11l1 fusion protein was constructed by placing the mTcp11l1 coding region into pEGFP-N1 vector and transferred into HEK293 cells. And then the subcellular location of the transferred protein was examined by fluorescence microscope. Subcellular localization indicated that TCP11L1 protein only existed in cytoplasm. RT-PCR analysis showed that Tcp1111 was expressed extensively. Bi... |
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| Keywords: | Tcp11l1 gene subcellular localization expression analysis transmembrane analysis |
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