盐酸小檗碱对小鼠脾细胞的DNA损伤和氧化性损伤研究

为了探讨盐酸小檗碱对小鼠的DNA损伤和氧化性损伤。随机选取30只小鼠分成对照组以及7.5，15，30，60与120 mg?kg^-1实验组，处理后，应用小鼠脾细胞进行彗星实验与抗氧化酶实验。测定DNA损伤情况以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性以及丙二醛（MDA）含量变化。对盐酸小檗碱的DNA损伤与氧化性损伤作用进行比较研究。研究结果表明：彗星实验中，随着盐酸小檗碱浓度的增加，尾部DNA含量、尾长与尾矩均增加，与阴性对照组相比差异有统计学意义（p<0.05或p<0.01），且呈剂量-效应关系；超氧化物歧化酶(SOD)与过氧化氢酶(CAT)活性随盐酸小檗碱剂量增加逐渐降低，丙二醛(MDA)含量明显下降，过氧化物酶(POD)活性在7.5 mg?kg^-1时上升，而后逐渐下降。在60 mg?kg^-1和120 mg?kg^-1时，有极显著性差异（p<0.01）产生。由此可见，盐酸小檗碱对小鼠脾细胞有一定的损伤作用，能够引起小鼠脾细胞的DNA损伤和氧化性损伤。

DNA damage and Oxidation damage of berberine hydrochloride on spleen of mice

To study the DNA damage and oxidation damage of bererbine hydrochloride. Thirty mice were divided into the controls, 7.5, 15, 30, 60 and 120 mg?kg^-1 berberine to determine the DNA damage in vitro by comet assay. Then the activity of catalase (CAT), superoxide dismutase (SOD),peroxidase (POD) and malondialdehyde (MDA) were measured. The results shown that compared with the controls, tail DNA content(%), tail length and tail moment went up respectively in dose-effect manner following the rising berberine hydrochloride, the change tendency showed significant differences (p<0.05 or p<0.01); As the content of berberine hydrochloride increased, the activities of dismutase (SOD), catalase (CAT) were gradually decreased and the concentration of malondialdehyde (MDA) was gradually increased.Peroxidase (POD) activity with 7.5 mg?kg^-1 berberine hydrochloride increased comparing to the controls and then gradually decreased over the course of the experiment. The statistically significant differences (p<0.05) were observed at the dose of 60 and 120 mg?kg^-1. The berberine hydrochloride could cause DNA damage and oxidation damage of mice spleen cells.