| 用铬革渣制备L-脯氨酸等六种氨基酸 |
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| 引用本文： | 蒋挺大,张春萍.用铬革渣制备L-脯氨酸等六种氨基酸[J].环境科学,1989,10(4):17-20,85. |
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| 作者姓名： | 蒋挺大 张春萍 |
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| 作者单位： | 中国科学院生态环境研究中心
(蒋挺大)，中国科学院生态环境研究中心(张春萍) |
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| 摘 要： | 铬革渣经碱性处理提取蛋白质,用6mol HCl水解,活性炭脱色,调节pH,由732~#阳离子交换树脂初步分离,得到的脯氨酸组分再由717#阴离子交换树脂分离,所得L-脯氨酸洗脱液减压蒸发浓缩,用无水乙醇结晶,得色谱纯L-脯氨酸。在732~#阳离子交换树脂上最后流出的是精氨酸,经处理得色谱纯L-精氨酸盐酸盐。在717~#阴离子交换树脂上分离可分别获得L-谷氨酸、L-天门冬氨酸、L-丙氨酸和甘氨酸。
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| 关 键 词： | 铬革渣 L－脯氨酸 氨基酸 |
| 收稿时间： | 9/5/1988 12:00:00 AM |
Reclamation of L-Proline and Other Amino-Acids from Chrome Leather Scraps |
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| Jiang Tingda and Zhang Chunping.Reclamation of L-Proline and Other Amino-Acids from Chrome Leather Scraps[J].Chinese Journal of Environmental Science,1989,10(4):17-20,85. |
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| Authors: | Jiang Tingda and Zhang Chunping |
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| Institution: | Research Center for Eco-Environmental Sciences, Academia Sinica, Beijing;Research Center for Eco-Environmental Sciences, Academia Sinica, Beijing |
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| Abstract: | The scraps of chrome leather is a tanning waste. The process of reclaiming six amino-acids was operated as follows: de-chroming of the scraps was adopted by basic hydrolysis with calcium oxide, and the protein extracted; the protein was hydrolyzed with 6 mol HC1; the hydroly-sate was declorized with activated carbon; then separated by 732 cation exchange resin (H form) and 717 anion exchange resin (OH form) respectively. L-Arg, L-Pro, L-Asp, L-Ala and Gly were obtained. |
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