Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey) |
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Authors: | Cigdem Alev Ozel Khalid Mahmood Khawar Semra Mirici Sebahattin Ozcan Orhan Arslan |
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Institution: | (1) Department of Biology Education, Faculty of Education, Gazi University, Besevler, 06000 Teknikokullar, Ankara, Turkey;(2) Institute of Biotechnology, Central Laboratory of Biotechnology, University of Ankara, Behind Rektorluk, 06000 Tandogan, Ankara, Turkey;(3) Department of Primary Education, Antalya, Turkey;(4) Department of Field Crops, Faculty of Agriculture, University of Ankara, 06110 Diskapi, Ankara, Turkey |
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Abstract: | Habitat destruction has resulted in the extinction of many plant species from the earth, and many more face extinction. Likely, the annual endemic Mediterranean knapweed (Centaurea tchihatcheffii) growing in the Golbasi district of Ankara, Turkey is facing extinction and needs urgent conservation. Plant tissue culture, a potentially useful technique for ex situ multiplication, was used for the restoration of this ill-fated plant through seed germination, micropropagation from stem nodes, and adventitious shoot regeneration from immature zygotic embryos. The seeds were highly dormant and very difficult to germinate. No results were obtained from the micropropagation of stem nodes. However, immature zygotic embryos showed the highest adventitious shoot regeneration on Murashige and Skoog (MS) medium, containing 1 mg l−1 kinetin and 0.25 mg l−1 NAA. Regenerated shoots were best rooted on MS medium containing 1 mg l−1 IBA and transferred to the greenhouse for flowering and seed set. As such, the present work is the first record of in vitro propagation of critically endangered C. tchihatcheffii, using immature zygotic embryos, and is a step forward towards conservation of this indigenous species. |
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Keywords: | Centaurea tchihatcheffii Seed dormancy Micropropagation Zygotic embryos Adventitious shoot regeneration |
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