高效厌氧氨氧化颗粒污泥的动力学特性

通过运行厌氧氨氧化上流式厌氧污泥床(upflow anaerobic sludge blanket,UASB)反应器,培育获得了高效厌氧氨氧化颗粒污泥(high-rate ANAMMOX granules,HAG).以絮状厌氧氨氧化污泥(flocculent ANAMMOX sludge,FAS)作为对照,采用批次试验研究了HAG的动力学特性.结果表明,HAG的比厌氧氨氧化活性(specific ANAMMOX activity,SAA)为2.63kg.(kg.d)-1,是FAS的2.5倍;对基质氨和亚硝酸盐的半速率常数(KS)分别为17 mg.L-1和19 mg.L-1,低于FAS的对应值;对氨、游离氨和亚硝酸盐的抑制常数(KI)分别为11 679、505和735 mg.L-1,远远高于FAS的对应值.HAG具有优良的动力学性质,可使其在不利环境中竞争基质,这对新型的厌氧氨氧化菌种流加工艺具有积极意义.

Kinetic Characteristics of High-rate ANAMMOX Granules

The high-rate ANAMMOX granules (HAG) were cultivated in an ANAMMOX upflow anaerobic sludge blanket (UASB) reactor. The kinetic characteristics of the HAG were investigated through batch tests, using flocculent ANAMMOX sludge (FAS) as a control. The results suggested that the specific ANAMMOX activity (SAA) of the HAG reached as high as 2.63 kg ·(kg ·d)^-1, which was 2.5 times of that of FAS. The half saturation constants for ammonium and nitrite of the HAG were 17 mg ·L^-1 and 19 mg ·L^-1, respectively, both were lower than those of the FAS. The half inhibition constants of ammonium, free ammonia and nitrite were 11679, 505 and 735 mg ·L^-1, respectively, which were far higher than the corresponding values of the FAS. The better kinetic performance of the HAG contributed to the successful competition of substrates under tough conditions, which was helpful for the novel biomass supplementation process for enhancement of the ANAMMOX performance.