| 石油降解菌株G5 16S rDNA全序列的系统学分析 |
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| 引用本文： | 洪斌,王丽非.石油降解菌株G5 16S rDNA全序列的系统学分析[J].应用与环境生物学报,2004,10(3):363-365. |
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| 作者姓名： | 洪斌 王丽非 |
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| 作者单位： | 中国医学科学院医药生物技术研究所,北京,100050 |
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| 摘 要： | 用PCR方法扩增了一株石油降解菌株G5的16S rRNA基因全序列，并对其进行了克隆和测序．对该序列在GenBank中的BLAST结果表明，所有与该序列高度同源的序列都是假单胞菌的16S rRNA基因．其中假单胞菌的代表菌株Pseudomonas aeruginosa，P．fluoroscens，P .putida，P.syringae的16S rRNA基因序列与G5的16S rRNA基因序列同源性分别为93．4％，98．4％，96．3％，97．5％．对G5和其他39株假单胞菌的16S rRNA基因序列进行聚类分析，获得的系统发育树与RDP(Ribosomal Database Project)报道的系统发育树基本一致，其中菌株G5与5株P．chlororaphis聚类在一起．图2参7
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| 关 键 词： | 16S rDNA 石油 微生物降解 假单胞菌 |
| 修稿时间： | 2003年5月9日 |
ANALYSIS OF 16S rDNA SEQUENCE OF A PETROLEUM-DEGRADING STRAIN G5 |
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| Abstract: | The 16S rRNA gene of bacteria strain G5, a petroleum degrading strain, was amplified, cloned and sequenced. BLAST of the sequence in GenBank indicated that the species with close similarity to G5 were from genus Pseudomonas only. The similarity values of 16S rDNA sequence between strain G5 and typical species P. aeruginosa , P. fluoroscens , P. putida and P. syringae were 93.4%, 98.4%, 96.3% and 97.5%, respectively. The phylogenetic tree was produced from 16S rDNA of G5 and other 39 Pseudomonas species using 16S rDNA of E. coli as outgroup. Strain G5 was clustered with 5 strains of P. chlororaphis . Fig 2, Ref 7 |
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| Keywords: | S rDNA petroleum degradation Pseudomonas |
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