邻苯二甲酸酯对浅层含水层沉积物微生物多样性的影响

在模拟浅层地下水低温、缺氧的环境条件下,利用BIOLOG ECO微平板法研究了经不同浓度邻苯二甲酸正丁酯(DBP)处理的江汉平原浅层含水层沉积物中微生物的数量、整体活性、多样性指数以及代谢功能多样性.结果表明,微生物的数量、整体活性随DBP浓度不同有差异;丰富度指数、Shannon指数与对照相比差异均不显著,而Simpson指数和McIntosh指数差异显著.低浓度DBP(<400mg/kg)可以抑制部分微生物的生长,而促进与DBP代谢有关的微生物活性;高浓度DBP(796mg/kg)则抑制了微生物数量,破坏物种的均一度,最终导致沉积物中微生物功能多样性发生改变而产生代谢变异性.

Effect of di-n-butyl phthalate (DBP) on the microbial diversity in shallow aquifer sediment

Impact of di-n-butyl phthalate(DBP),an endocrine-disrupting chemical,on microbial community diversities and activities in shallow aquifer sediments in Jianghan plain were investigated in batch cultures via BIOLOGECO microplates.Sediments were amended with different concentrations of DBP varied from 0 to 796mg/kg and incubated under a condition of hypothermia-hypoxia which was commonly found in subsurface aquifer.Average well colour development (AWCD) was measured with mircosation biolog periodically.It was observed that the microbial populations and activities vailed with DBP concentrations.With the variation of DBP concentrations no obvions difirerenge was detected among the richness (R) and Shannon's index (H),while remarkable differences was found among the Simpson's indices and McIntosh's indices of the control and sediments amended with DBP (P<0.05).The microbial activity which might potentially related to DBP degradation was enhanced by DBP with low concentration (<400mg/kg).However,with higher concentrations,(≥796mg/kg),DBP grossly inhibited microbial growth and metabolism as manifested by the reduction in microbial count,and destabilized the natural balance of microbial communities.