| 污泥好氧颗粒化过程中微生物群落结构的演变与分析 |
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| 引用本文： | 张斌,邱志刚,金敏,陈照立,谌志强,王景峰,李君文. 污泥好氧颗粒化过程中微生物群落结构的演变与分析[J]. 环境工程学报, 2011, 5(10): 2369-2374 |
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| 作者姓名： | 张斌 邱志刚 金敏 陈照立 谌志强 王景峰 李君文 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所,天津300050／天津市环境与食品安全风险监控技术重点实验室,天津300050 |
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| 基金项目： | 国家自然科学基金资助项目(50908227)；国家高技术研究发展计划（863）项目(2009AA06Z312) |
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| 摘 要： | 为了揭示颗粒污泥形成过程中微生物群落结构多样性的演变过程,以人工配水为进水,在SBR中采用厌氧/好氧循环的手段成功培育出具有聚磷特性的颗粒污泥,利用基于16S rDNA的PCR-DGGE技术获得了微生物群落的DNA特征指纹图谱,对条带进行了统计分析和切胶测序,并建立了系统发育树。结果表明,污泥沉降性能的改善要先于颗粒污...
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| 关 键 词： | 颗粒污泥 微生物群落结构 聚磷菌 微生物生态学 |
Evolution and analysis of microbial community structure during aerobic sludge-granulation |
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| Zhang Bin,Qiu Zhigang,Jin Min,Chen Zhaoli,Chen Zhiqiang,Wang Jingfeng and Li Junwen. Evolution and analysis of microbial community structure during aerobic sludge-granulation[J]. Techniques and Equipment for Environmental Pollution Control, 2011, 5(10): 2369-2374 |
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| Authors: | Zhang Bin Qiu Zhigang Jin Min Chen Zhaoli Chen Zhiqiang Wang Jingfeng Li Junwen |
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| Affiliation: | Zhang Bin1,2 Qiu Zhigang1,2 Jin Min1,2 Chen Zhaoli1,2 Chen Zhiqiang1,2 Wang Jingfeng1,2 Li Junwen1,2 (1.Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China,2.Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety,China) |
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| Abstract: | In order to reveal the succession procedure of microbial community diversity during sludge granulation,polyphosphate-accumulating granules were successfully cultured during the anaerobic/aerobic cycle with artificially influent water.PCR-amplified 16S rDNA and denaturing gradient gel electrophoresis(DGGE) were applied to obtain DNA characterized fingerprint profile of the microbial communities.The bands in the gel were analyzed by statistical methods and excided from the gel for sequencing,and the phylogene... |
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| Keywords: | granular sludge microbial community structure polyphosphate-accumulating organisms(PAO) microbial ecology |
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