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PVA降解混合菌系生长和产酶的营养条件分析
引用本文:崔双科,郭雅妮,赵倩楠,郭战英,张巧绸. PVA降解混合菌系生长和产酶的营养条件分析[J]. 环境工程学报, 2011, 5(9): 2144-2150
作者姓名:崔双科  郭雅妮  赵倩楠  郭战英  张巧绸
作者单位:1. 陕西省现代建筑设计研究院,西安,710048
2. 西安工程大学环境与化学工程学院,西安,710048
3. 陕西省礼泉县环境保护局,礼泉,713200
基金项目:陕西省科技厅科学技术研究发展计划项目(2010K02-10)
摘    要:研究碳源、氮源以及不同金属离子对PVA降解混合菌系生长和产酶的影响。结果表明,混合菌系生长过程中无机氮源优于有机氮源;补充碳源可促进混合菌系的生长,但酶活比不添加时稍有降低。金属离子对酶活影响的正交实验表明Mg2+浓度影响最大,其次是Fe2+和K+浓度,Ca2+、Mn2+、Zn2+和Cu2+浓度影响较小;最佳浓度分别为Mg2+0.05 g/L、Fe2+0.04 g/L、K+2 g/L和Ca2+0.05 g/L。

关 键 词:聚乙烯醇  降解酶  碳源  氮源  金属离子

Aanalysis on the nutritional condition of growth and enzyme production for PVA degradation mixed strains
Cui Shuangke,Guo Yani,Zhao Qiannan,Guo Zhanying and ZhangQiaoChou. Aanalysis on the nutritional condition of growth and enzyme production for PVA degradation mixed strains[J]. Techniques and Equipment for Environmental Pollution Control, 2011, 5(9): 2144-2150
Authors:Cui Shuangke  Guo Yani  Zhao Qiannan  Guo Zhanying  ZhangQiaoChou
Affiliation:Cui Shuangke1 Guo Yani2 Zhao Qiannan2 Guo Zhanying1 Zhang Qiaocou3(1.Shaanxi Modern Architectural Design Research Institute,Xi'an 710048,China,2.College of Environmental and Chemistry Engineering,Xi' an Polytechnic University,3.Environmental Protection Agency of Liquan County in Shaanxi Province,Liquan 713200,China)
Abstract:The influence for growth and enzyme production of different concentration of carbon and nitrogen source and metal ions was investigated in a mixed strains.The results indicated that the inorganic nitrogen sources were better than organic nitrogen sources during the growth of mixed strains.And other carbon sources could promote the growth of microorganism but restrain the activity of PVA degrading enzyme.The orthogonal test results of metal ions on enzyme activity indicated that the concentration of Mg2+ was...
Keywords:PVA  degrading enzyme  carbon source  nitrogen source  metal ions  
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