均匀设计用于研究硝基苯衍生物对青海弧菌Q67的联合毒性

实验设计在混合物毒性评估与预测中起着非常重要的作用.应用微板毒性测试方法测定了7种硝基苯衍生物对青海弧菌Q67的发光抑制毒性,硝基苯、邻氯硝基苯、间氯硝基苯、对氯硝基苯、间硝基苯胺、对硝基苯胺和对硝基甲苯的-lg EC50值(EC50的单位为mol/L)分别为2.66,3.22,3.30,3.29,2.94,4.22和3.39;引入均匀实验设计方法,在单个硝基苯衍生物剂量-效应关系基础上构建不同效应浓度下的10个混合物,同样应用微板毒性测试方法测定其对Q67的毒性,应用剂量加和(DA)与独立作用(IA)原理建立了混合物毒性的评估与预测模型.结果表明:与等效应浓度比法相比,均匀实验设计构建的混合物浓度配比,具有三维浓度分布特征,可在更大范围内考察各种可能的昆合物类型,更加接近于实际环境体系.

Use of Uniform Design to Evaluate the Joint Toxicity of Nitrobenzene Derivativesto Vibrio qinghaiensis sp.-Q67

Experimental design plays an important role in the study of mixture toxicity. Using the microplate toxicity test method, luminescence inhibition toxicity of seven nitrobenzene derivatives (NBDs) to Vibrio qinghaiensis sp.-Q67was determined. The-lg EC₅₀ values (EC₅₀ unit: mol/L) of sevenNBDs, i.e. nitrobenzene, 1-chloro-2-nitrobenzene, 1-chloro-3-nitrobenzene, 1-chloro-4-nitrobenzene, 3-nitroaniline, 4-nitroaniline and 4-Nitrotoluene, are 2.66, 3.22, 3.30, 3.29, 2.94, 4.22 and 3.39, respectively. On the basis of the dose-response relationships of seven NBDs, the uniform design was used to construct 10 NBD mixtures with different effect concentration. Similarly, applying microplate toxicity test method the toxicities of 10 NBD mixtures to Q67 were determined. The models for evaluating and predicting the mixture toxicity were developed using the dose additionand independent action principles. The results showed that compared to the equivalent-effect concentration ratio method, the concentration ratios of various NBD components in the mixtures constructed by the uniform design were scattered over the three-dimensional concentration space, which can examine the possible mixture types in a greater scope, closer to the actual environmental system.