NADP+-isocitrat-dehydrogenase aus Idus idus (Pisces: Cyprinidae). II. Einfluß der temperatur auf substrat- und cosubstrataffinität |
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Authors: | Dr H Künnemann D Passia |
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Institution: | (1) Present address: Lehrstuhl für Vergleichende Physiologie und Tierpsychologie, Zoologisches Institut der Universität Kiel, Kiel, Germany (FRG) |
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Abstract: | Maximum substrate and cosubstrate affinity, as judged by the Michaelis constant (K
M
), of NADP+-dependent isocitrate dehydrogenase of pig heart (purchased from Boehringer, Mannheim, FRG) is attained at 37°C. If K
M
-values of substrate (Isocitrate, IC) and cosubstrate (NADP+) of NADP+-dependent isocitrate dehydrogenase (ICDH) of the white dorsal muscle of Idus idus L. is plotted against the experimental temperature (VT), W-shaped curves result. With increasing adaptation temperature (AT), there is a shift to increasing VT. It is suggested that the W-shaped curves are due either to the simultaneous presence of two multiple forms of the enzyme, or to the reversible temperature-dependent interconversion of one protein species. |
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Keywords: | |
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