邻苯二甲酸二(2-乙基已)酯(DEHP)对马氏珠母贝(Pinctada maetensii)胚胎发育的影响及DNA损伤

邻苯二甲酸二(2-乙基己)酯(DEHP)是一种常用的增塑剂,在环境中广泛存在。本研究以马氏珠母贝胚胎为研究对象,揭示DEHP短期胁迫对胚胎发育的影响及胚胎DNA损伤。结果显示:DEHP对D型面盘幼虫的发育有显著影响,同一浓度组的死亡率与时间呈正相关。在(4~32)mg/L浓度范围内,不同胁迫时间段,幼虫的死亡率随染毒浓度升高而上升,表现出明显的剂量-效应关系,说明DEHP暴露能影响马氏珠母贝D型面盘幼虫的发育,并可能对其种群结构产生不利影响。DEHP胁迫还能对胚胎 DNA造成明显损伤,随着DEHP染毒浓度的增大,彗星尾长(TL)、尾矩(OTM)明显增加,尾部DNA含量(T-DNA)上升,3个指标表现出良好的相关性,DNA的损伤与暴露浓度间存在明显的剂量-效应关系。研究结果表明马氏珠母贝D型面盘幼虫的发育和形态学变化、胚胎细胞的DNA的损伤可以作为反映海洋环境中DEHP污染早期预警的敏感生物标志物。

Effects of DEHP on embryonic development and DNA damage of Pinctada maetensii

Di-2-ethylhexyl phthalate (DEHP) is used as a plasticizer commonly and exists widely in environment.The effects of early D-shaped veliger larvae development and embryonic DNA damage of Pinctada martensii exposure to DEHP were studied in this report.The results showed that DEHP had a significant effect on the development of D-shaped veliger larvae.There was a positive correlation between mortality rate of D-shaped veliger larvae and exposure time of DEHP in a same concentration group.In the concentration groups of (4~32 )mg/L,there was a significant dose-response relationship between the larval mortality rate and exposure concentrations at different exposure time.It can be concluded that DEHP exposure caused adverse effects on the development of D-shaped veliger larvae and may also influence the population structure of Pinctada martensii.DEHP exposure induced DNA damage of embryo cells significantly.There was a good dose-effect relationship between DNA migration and DEHP concentrations.Tail length (TL),tail DNA content (T-DNA) and olive tail moment (OTM) increased significantly with exposure concentration.The results indicated that developmental and morphological changes of D-shaped veliger larvae and the DNA damages in embryo cells of Pinctada martensii could be used as sensitive biomarkers of DEHP exposure in the marine environment.