Biosurfactant-producing strains in enhancing solubilization and biodegradation of petroleum hydrocarbons in groundwater |
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Authors: | Hong Liu Hang Wang Xuehua Chen Na Liu Suriguge Bao |
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Affiliation: | 1. Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun, 130021, People’s Republic of China 2. School of Resources and Environment Engineering, Jilin Institute of Chemical Technology, Jilin, Jilin, 132022, People’s Republic of China
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Abstract: | Three biosurfactant-producing strains designated as BS-1, BS-3, and BS-4 were screened out from crude oil-contaminated soil using a combination of surface tension measurement and oil spreading method. Thin layer chromatography and infrared analysis indicated that the biosurfactants produced by the three strains were lipopeptide, glycolipid, and phospholipid. The enhancement of solubilization and biodegradation of petroleum hydrocarbons in groundwater employing biosurfactant-producing strains was investigated. The three strain mixtures led to more solubilization of petroleum hydrocarbons in groundwater, and the solubilization rate was 10.5 mg l?1. The combination of biosurfactant-producing strains and petroleum-degrading strains exhibited a higher biodegradation efficiency of 85.4 % than the petroleum-degrading strains (71.2 %). Biodegradation was enhanced the greatest with biosurfactant-producing strains and petroleum-degrading strains in a ratio of 1:1. Fluorescence microscopy images illustrate that the oil dispersed into smaller droplets and emulsified in the presence of biosurfactant-producing strains, which attached to the oil. Thus, the biodegradation of petroleum hydrocarbons in groundwater was enhanced. |
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