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Identification of five partial ABC genes in the liver of the Antarctic fish Trematomus bernacchii and sensitivity of ABCB1 and ABCC2 to Cd exposure |
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| Authors: | Sara Zucchi Ilaria Corsi Shannon Mala Bard Silvano Focardi |
| Institution: | a Department of Environmental Sciences “G. Sarfatti”, University of Siena, Via Mattioli 4, 53100 Siena, Italy b UFZ - Helmholtz Centre for Environmental Research, Permoserstr. 15, D-04318 Leipzig, Germany c Environmental Programmes, Dalhousie University, 1355 Oxford Street, Life Science Centre, Room 820, Halifax, Nova Scotia, Canada B3H 4J1 d Department of Biochemistry, Biology and Genetics, Polytechnic University of Marches, Ancona, Italy |
| Abstract: | Several ABC transporters have been characterized from many aquatic organisms, but no information is yet available for Antarctic fish. The aim of this work was to identify the expression of genes for ABC proteins in Trematomus bernacchii, a bioindicator species of the Southern Ocean. Partial cDNA sequences of ABCB1, ABCC1, ABCC2, ABCC4 and ABCC9 were cloned from liver. Using RACE technology, 3.5 and 2.2 kb contigs were obtained for ABCB1 and ABCC2. Considering the elevated natural bioavailability of cadmium at Terra Nova Bay, responsiveness of ABCB1 and ABCC2 to this element was investigated under laboratory conditions. ABCB1 and ABCC2 mRNA levels were approximately four-fold higher in Cd-exposed fish compared to the controls. Induction of ABCB1 protein was also found by western blot. This study provides the first identification of five ABC genes in the liver of an Antarctic key species, some of which may be involved in cellular detoxification. |
| Keywords: | ABC transporters Liver Cadmium Trematomus bernacchii Antarctica |
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