|
|||||
|
|
Sublethal toxicity of nano-titanium dioxide and carbon nanotubes in a sediment dwelling marine polychaete |
|
| Authors: | Tamara Galloway Ceri Lewis Blair D Johnston Francesco Regoli |
| Institution: | a School of Biosciences, Hatherly Laboratories, University of Exeter, EX4 4PS, Exeter, Devon, UK b Università Politecnica delle Marche, Institute of Biology and Genetics, Via Ranieri, Monte Dago,60121 Ancona, Italy c School of Physics, Stocker Road, University of Exeter, Devon EX4 4QL, UK |
| Abstract: | The ecotoxicology of manufactured nanoparticles (MNPs) in estuarine environments is not well understood. Here we explore the hypothesis that nanoTiO2 and single walled nanotubes (SWNT) cause sublethal impacts to the infaunal species Arenicola marina (lugworm) exposed through natural sediments. Using a 10 day OECD/ASTM 1990 acute toxicity test, no significant effects were seen for SWNT up to 0.03 g/kg and no uptake of SWNTs into tissues was observed. A significant decrease in casting rate (P = 0.018), increase in cellular damage (P = 0.04) and DNA damage in coelomocytes (P = 0.008) was measured for nanoTiO2, with a preliminary LOEC of 1 g/kg. Coherent anti-stokes Raman scattering microscopy (CARS) located aggregates of TiO2 of >200 nm within the lumen of the gut and adhered to the outer epithelium of the worms, although no visible uptake of particles into tissues was detected. |
| Keywords: | Ecotoxicology Arenicola marina Nanoparticle Coherent anti-stokes Raman microscopy Genotoxicity |
| 本文献已被 ScienceDirect 等数据库收录！ | |