Methanotrophs and methanotrophic activity in engineered landfill biocovers |
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| Authors: | S. Ait-Benichou Louis-B. Jugnia Charles W. Greer Alexandre R. Cabral |
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| Affiliation: | 1. Faculty of Engineering, Civil Engineering Department, Université de Sherbrooke, 2500 Boulevard Université, Sherbrooke, Québec, Canada J1K 2R1;2. Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Ave., Montreal, Quebec, Canada H4P 2R2;1. KERMIT, Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University, Coupure links 653, 9000 Gent, Belgium;2. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium;3. Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Ghent University, Coupure links 653, B-9000 Gent, Belgium;4. BCCM/LMG Bacteria Collection, Gent, Belgium;1. Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China;2. School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;3. State Environmental Protection Key Laboratory of Microorganism Application and Risk Control(MARC), Tsinghua University, Beijing 100084, PR China;4. School of Environment, Tsinghua University, Beijing 100084, PR China |
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| Abstract: | The dynamics and changes in the potential activity and community structure of methanotrophs in landfill covers, as a function of time and depth were investigated. A passive methane oxidation biocover (PMOB-1) was constructed in St-Nicéphore MSW Landfill (Quebec, Canada). The most probable number (MPN) method was used for methanotroph counts, methanotrophic diversity was assessed using denaturing gradient gel electrophoresis (DGGE) fingerprinting of the pmoA gene and the potential CH4 oxidation rate was determined using soil microcosms. Results of the PMOB-1 were compared with those obtained for the existing landfill cover (silty clay) or a reference soil (RS). During the monitoring period, changes in the number of methanotrophic bacteria in the PMOB-1 exhibited different developmental phases and significant variations with depth. In comparison, no observable changes over time occurred in the number of methanotrophs in the RS. The maximum counts measured in the uppermost layer was 1.5 × 109 cells g dw?1 for the PMOB-1 and 1.6 × 108 cells g dw?1 for the RS. No distinct difference was observed in the methanotroph diversity in the PMOB-1 or RS. As expected, the potential methane oxidation rate was higher in the PMOB-1 than in the RS. The maximum potential rates were 441.1 and 76.0 μg CH4 h?1g dw?1 in the PMOB and RS, respectively. From these results, the PMOB was found to be a good technology to enhance methane oxidation, as its performance was clearly better than the starting soil that was present in the landfill site. |
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