|
|||||
|
|
Arsenic concentration in topsoil of central Chile is associated with aberrant methylation of P53 gene in human blood cells: a cross-sectional study |
|
| Authors: | Madrid Eva Gonzalez-Miranda Isabel Muñoz Sergio Rejas Carolina Cardemil Felipe Martinez Felipe Cortes Juan Pablo Berasaluce Maite Párraga Mario |
| Institution: | 1.Interdisciplinary Centre for Health Studies (CIESAL) - Escuela de Medicina, Universidad de Valparaíso, Vi?a del Mar, Valparaíso, Chile ;2.Centro Regional de Investigación e Innovación para la Sostenibilidad de la Agricultura y los Territorios Rurales (Ceres), Quillota, Valparaíso, Chile ;3.Pontificia Universidad Católica de Valparaíso, Vicerrectoría de Investigación y Estudios Avanzados, Valparaíso, Chile ;4.Department of Public Health–CIGES, Universidad de La Frontera, Temuco, Chile ;5.Department of Surgery, University Hospital Regensburg, Regensburg, Germany ;6.Department of Basic and Clinical Oncology, School of Medicine, Universidad de Chile, Santiago, Chile ;7.Facultad de Medicina, Escuela de Medicina, Universidad Andrés Bello, Vi?a del Mar, Chile ;8.Laboratorio de Biología Molecular, Centro de Investigaciones Biomédicas, Universidad de Valparaíso, Valparaíso, Chile ;9.Mental Health Unit, Hospital Carlos van Buren, Valparaiso, Chile ; |
| Abstract: | Gene expression can be modified in people who are chronically exposed to high concentrations of heavy metals. The soil surrounding the Ventanas Industrial Complex, located on the coastal zone of Puchuncaví and Quintero townships (Chile), contain heavy metal concentrations (As, Cu, Pb, Zn, among others) that far exceed international standards. The aim of this study was to determine the potential association of the heavy metals in soils, especially arsenic, with the status of methylation of four tumor suppressor genes in permanent residents in those townships. To study the methylation status in genes p53, p16, APC, and RASSF1A, we took blood samples from adults living in areas near the industrial complex for at least 5 years and compared it to blood samples from adults living in areas with normal heavy metal concentrations of soils. Results indicated that inhabitants of an area with high levels of heavy metals in soil have a significantly higher proportion of methylation in the promoter region of the p53 tumor suppressor gene compared with control areas (p-value: 0.0035). This is the first study to consider associations between heavy metal exposure in humans and aberrant DNA methylation in Chile. Our results suggest more research to support consistent decision-making on processes of environmental remediation or prevention of exposure. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |