Influence du volume d'inoculum dans la quantification des virus selon deux techniques comparees: Plages et N.P.P. utilisant un grand nombre d'inoculum par dilution |
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Authors: | B Hugues Ch Pietri |
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Institution: | 1. Laboratoire d''Hygiène de la Ville de Nice, 8, rue de l''Hôtel des Postes, 06000 Nice, France;2. Unité 40 de l''I.N.S.E.R.M., 1, avenue Jean Lorrain 06300 Nice, France |
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Abstract: | Viral particles can be quantified by two different titration methods which provided the same results: counting plates in solid medium and MPN method, using a large number of inocula for each dilution, in liquid medium. The accuracy of the two methods is a function of the volume of the inoculum used. The width of the confidence interval increases when the volume of the inoculum decreases. |
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