高效氨氧化菌群富集、驯化及其动态变化规律分析

利用选择性培养基对氨氧化菌群进行了连续驯化,得到了氨氮去除效率稳定的氨氧化菌群。采用平板菌落计数法结合聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术考察了氨氧化菌群在连续传代过程中数量及群落结构的动态变化,并考察了pH值、碳源(HCO3-)浓度和氨氮浓度等因素对氨氧化菌群去除氨氮效率的影响。结果表明,通过连续驯化,氨氧化菌的比例由最初的1.8%提高到了31.3%。在碳源浓度为1.5 mg/L,NH4+-N初始浓度为200 mg/L,pH值为8的条件下,菌群对氨氮的去除率达99%以上。

Domesticate and enrichment of high concentration of ammonia oxidation bacteria and its dynamic change analysis

Ammonia oxidation bacteria(AOB) with stable ammonia-nitrogen removal were obtained by continuous domestication using enrichment culture medium.The dynamic changes of bacterial counts and community structure were analyzed by direct plate counting and polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) technique.Meanwhile,the effects of pH value,and concentrations of carbon source(HCO-3) and ammonia-nitrogen on ammonia removal efficiency were studied.The experimental results indicated that the ratio of AOB to the total number of bacteria in the system increased from 1.8% to 31.3% through continuous domestication.The removal rate of ammonia-nitrogen reached more than 99% using the obtained AOB with 1.5 mg/L of carbon source,200 mg/L of initial NH+4-N concentration at pH 8.