Inheritance of a nuclear DNA polymorphism assayed in single bivalve larvae |
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| Authors: | H B S M Côrte-Real P W H Holland D R Dixon |
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| Institution: | (1) Department of Zoology, University of Oxford, South Parks Road, OX1 3PS Oxford, England;(2) Plymouth Marine Laboratory, Citadel Hill, PL1 2PB Plymouth, Devon, England;(3) Present address: Department of Pure and Applied Zoology. School of Animal and Microbial Sciences, University of Reading, Whiteknights, RG6 2AJ Reading, England |
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| Abstract: | We examined the inheritance of theMytilus edulis CaM-1 Intron 3 locus, a non-coding DNA locus with two potentially neutral length-variants. The polymerase chain reaction (PCR) was used to determine theCaM-1 genotype for 799 larvae obtained from 11 laboratory crosses. Larvae were typed singly only 8 to 24 h after fertilization. We find evidence that each allele can be inherited from either sex, that there are no barriers to fertilization between gametes of different genotypes, and that most larvae have genotypes compatible with Mendelian inheritance of the locus. Deviations from expected genotype frequencies were found in some crosses; we suggest that a contributing factor is aneuploidy in early larvae, but a major cause is more likely to be selection at a locus linked toCaM-1, occurring under the artificial laboratory culture conditions. This study demonstrates the feasibility of applying molecular genetic techniques to early larval stages of marine bivalves, and presents a new non-destructive biopsy method for DNA analysis from living adult mussels. |
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