| 改良MTT法在鱼类细胞毒性实验中的使用条件 |
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| 引用本文： | 王宇飞,邬红娟,文琛,刘光虹.改良MTT法在鱼类细胞毒性实验中的使用条件[J].环境科学与技术,2009,32(2). |
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| 作者姓名： | 王宇飞 邬红娟 文琛 刘光虹 |
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| 作者单位： | 华中科技大学环境科学与工程学院,湖北武汉,430074 |
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| 基金项目： | 农业部引进国际先进农业科学技术计划(948计划)，湖北省科技攻关课题 |
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| 摘 要： | 文章利用斑点叉尾鮰鱼卵巢细胞(CCO)、鲤鱼上皮癌细胞(EPC)、草鱼肾细胞(CIK)探讨改良四噻唑蓝(MTT)比色法在测定污染物对鱼类细胞急性毒性实验中的最佳条件。通过实验发现其最佳实验条件为570nm波长下,MTT作用4h,MTT终浓度1mg/mL,最佳接种量的确定因每种细胞的特性而不同,生长速度快的细胞接种量较少。
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| 关 键 词： | 改良MTT法 鱼类细胞 体外急性毒性 |
Optimum Conditions of Improved MTT Assay for Estimating in Vitro Acute Cytotoxicity in Different Fish Cells |
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| WANG Yu-fei,WU Hong-juan,WEN Chen,LIU Guang-hong.Optimum Conditions of Improved MTT Assay for Estimating in Vitro Acute Cytotoxicity in Different Fish Cells[J].Environmental Science and Technology,2009,32(2). |
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| Authors: | WANG Yu-fei WU Hong-juan WEN Chen LIU Guang-hong |
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| Institution: | School of Environmental Science and Engineering;Huazhong University of Science and Technology;Wuhan 430074;China |
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| Abstract: | Tests were done to determine the optimum conditions of improved MTT assay for estimating in vitro acute cytotoxicity of fish cell through Channel Catfish Ovary(CCO)cell,Ctenpharyngoden Idellus Kindy(CIK)cell and Epithelioma Papulosum Cyprinid(EPC)cell. Results showed that under the optimum conditions of wavelength 570nm and incubation time 4h,MTT concentration was 1mg/mL,with inoculation cell number depended on characteristics of cell and increased with growth speed of the cell. |
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| Keywords: | improved MTT assay fish cell in vitro acute cytotoxicity |
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