氟化钠和氟乙酰胺对培养赤麂细胞姐妹染色单体互换和染色体畸变的影响

本文采用培养的赤麂细胞对氟化钠和氟乙酰胺进行致突变效应的研究.观察指标是染色体畸变率(％),姐妹染色单体交换率(SCEs)和细胞周期动力学变化.实验结果证明,氟化钠和氟乙酰胺在培养的赤麂细胞上都能引起染色体畸变率增高,促使SCEs增加和延迟细胞周期.因此,本工作实验结果支持了部份文献中氟化物是一种致突变物的结论.

EFFECT OF SODIUM FLUORIDE AND FLUOROACETAMIDE ON SISTER CHROMATID EXCHANGES AND CHROMOSOMAL ABERRATIONS IN CULTURED RED MUNTJAC (MUNTJACUS MUNTJAK) CELLS

Fluoride has been one of the environmental chemical contaminants and has long become an important subject of toxjcological study. Although many investigations and studies have been made and shown that acute, subacute or chronic exposure to fluoride can cause fluorosis in organisms, studies on fluoride mutagenicity are few, and there are three kinds of experimental results and point of view: 1. non-mutagenic, 2. mutagenic and, 3. synergistic or antagonistic effect with other chemicals in the combined test. Hence, chromosomal aberrations, sister chromatid exchanges (SCE), and cell cycle kinetics in the cultured cells of Red Muntjac in vitro were used as indices in studying the mutagenic effects of sodium fluoride and fluoroacetamide. The results showed that both sodium fluoride and fluoroacetamide can cause chromosomal breakage, increase SCE frequency and lag cell cycle, but fluoroacetamide can cause only chromosomal breakage and lag cell cycle. Obviously, mutagenicity of fluoride can thus be confirmed once more by the results.