高效降解黑臭废水细菌的筛选及鉴定

以筛选出能够高效降解黑臭废水的更多细菌为目的,从不同重污染水体和底泥中筛选获得了多种细菌,并对其降解能力进行检测。净化实验结果表明,筛选出的50多株细菌中有12株对废水COD有明显的降解效果,降解率在45%以上;有4株菌对废水氨氮有明显的降解效果,降解率在30%以上,其中4号菌株对COD和氨氮都有很高的降解能力。经16SrRNA基因鉴定,4号菌株为不动杆菌属,由单因子优化实验得出4号菌株生长的最适条件为:温度30℃,pH7.5,转速180r/min,培养时间为22h时,微生物达到稳定期。在此条件下,4号菌株对自然黑臭废水COD和氨氮的降解率可达到92%和72%。

SCREENING AND IDENTIFICATION OF EFFICIENT MICROORGANISM STRAINS CAPABLE OF DEGRADING BLACK AND ODOROUS WASTEWATER

In order to screen out efficient bacteria which could degrade black and odorous wastewater,more than 50 species of bacteria were isolated from different polluted sediments and sewage.The degradation of species could be detected.Purification experiment results showed that there were 12 isolated strains which had a good COD degradation rate,more than 45%,4 isolated strains which had a good ammonia nitrogen degradation rate,more than 15%.No.4 strain had a good degradation rat of ammonia nitrogen and COD.No.4 strain was identified as Acinetobacter sp.by 16S rRNA gen.The optimum conditions of No.4 strain growth ware as below:temperature is 30 ℃,pH was 7.5,speed was 180 r/min through single factor experiment.Training time was 22h,microbial reached stationary phase.Under these conditions,the degradation rate of ammonia nitrogen and COD for nature black and odorous wastewater could reach 92% and 72%.