In ovo nanoinjection of nonylphenol affects embryonic development of a transgenic see-through medaka (Oryzias latipes), olvas-GFP/STII-YI strain |
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| Institution: | 1. Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812–8581, Japan;2. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606–8502, Japan;3. Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki 444-8585 Aichi, Japan;4. Laboratory of Freshwater Fish Stocks, Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464–8601, Japan;1. Department of Environmental Science, Faculty of Science, Niigata University, Ikarashi, Niigata, 950-2181, Japan;2. Ushimado Marine Institute, Faculty of Science, Okayama University, Ushimado, Setouchi, 701-4303, Japan;3. Department of Bioscience, Faculty of Bioscience, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, 526-0829, Japan;1. Nicholas School of the Environment, Duke University, Durham, NC 27708-0328, United States;2. Center for the Environmental Implications of Nanotechnology, Duke University, Durham, NC 27708-0328, United States;3. Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China;1. Laboratory of Molecular Virology and Biotechnology, Institute of Biotechnology, National Cheng-Kung University, Tainan 701, Taiwan;2. Department of Life Sciences, National University of Kaohsiung, Kaohsiung 811, Taiwan;3. Laboratory of Marine Molecular Biology and Biotechnology, Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei 115, Taiwan;4. Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung 811, Taiwan |
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| Abstract: | We performed in ovo nanoinjection of 4-nonylphenol (NP) into embryos of a transgenic see-through medaka (Oryzias latipes), olvas-GFP/STII-YI strain, which has two genotypic sex markers, and examined the effects on development and sexual differentiation. The transgene consisted of a green fluorescent protein (GFP) gene fused to the regulatory region of the medaka vasa gene. Germ cell-specific GFP expression was visualized in the gonad through the transparent body wall of the living fish. The development of each embryo was observed after nanoinjection of 2.0, 10, 50, 125, or 250 ng of NP. NP administration caused significant higher mortality at ⩾50 ng egg−1 and inhibited embryonic development, including abnormal hatch and swim-up failure in all treatment groups except 10 ng egg−1 group. However, it did not cause adverse effects on germ cell proliferation by 10 d posthatch (dph) or sex differentiation of survivors by 100 dph. We concluded that single-dose in ovo exposure to nonylphenol affected embryonic development in the medaka but not gonadal development by 10 dph or sexual differentiation in adult fish by 100 dph. Although further investigations might be needed to elucidate the usefulness of nanoinjection of embryos of this strain, present study indicated that the nanoinjection model using olvas-GFP/STII-YI strain medaka has potential for use in evaluating the effects of chemicals on early development and sexual differentiation. |
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