固定化酶催化氧化去除水溶液中酚的研究

用海藻酸钙凝胶包埋辣根过氧化物酶制成固定化以过氧化氢为氧化剂，进行了固定化酶催化氧化去除水溶液中酚的实验研究。对于１００ｍｌ酚住房为１００ｍｇ／Ｌ的单一苯酚或邻氯基水溶液，控制条件为：３％海藻酸钠液３０ｍｌ，凝胶粒径１ｍｍ，固定化时间１ｈ，酶包埋量５０ｕ，反应时间１ｈ，ｐＨ６及过氧化氢加入量２ｍｍｏｌ，单一苯酚和单一 基酚水溶液的酚去除率可分别超过８０％和７０％。

Study on the Catalytic Removal of Phenols from Aqueous Solution by Immobilbe Enzyme

he removal of phenols from aqueous solution by immobilized enzyme catalytic oxidation was studied.The immobilized enzyme was prepared by containing horseradish peroxidase with calcium alginate gel,and hydrogen peroxide was used as oxidizing agent. For 100 ml phenol or oaminophenol aqueous solution containing 100 mg/L phenol, under the following appropriate conditions:3% sodium alginate solution 30 ml,gel particle diameter 1 mm, immobilization time 1h,containing amount of enzyme 50 u,reaction time 1h, pH 6 and hydrogen peroxide dose 2 mmol,the removal rates of phenol and oaminophenol exceeded 80% and 70% respectively.There was synergistic effect between various phenols in the treatment of mixed phenol aqueous solution.The treatment result after mixing different phenol wastewaters is better than their separate treatment result. The immobilized enzyme prepared has fair repeating usage performance.