土壤中磺胺二甲嘧啶赋存对水稻生长的影响

为探究土壤中残留抗生素对植物生长的影响,选取土壤中检出率较高的磺胺类抗生素磺胺二甲嘧啶作为外源污染物,分析水稻苗期和成熟期生长指标、根系和叶片生理生化指标、水稻各器官中抗生素残留量和富集转运因子的变化特性,并评价水稻籽粒中残留磺胺二甲嘧啶的健康风险.结果表明,磺胺二甲嘧啶对水稻株高和生物量的抑制作用存在于整个生长周期中,且苗期的影响大于生长成熟期,根部受到的影响大于苗部.苗期秧苗根系活力、硝酸还原酶活性和叶片叶绿素含量受到随抗生素含量增加而增大的抑制作用,而抗氧化酶活性的变化趋势表现出不同特征,具体为超氧化物歧化酶活性增强、过氧化氢酶和过氧化物酶先被激活后被抑制.磺胺二甲嘧啶在水稻各器官中的积累量表现为：根>叶>茎>籽粒,且水稻籽粒的抗生素风险评估结果显示EDI/ADI<0.1,不构成健康风险.生长成熟期土壤中磺胺二甲嘧啶对水稻富集因子和转运系数的影响大于苗期.综合磺胺二甲嘧啶对水稻的不利影响,在水稻种植中施加畜禽粪便作为有机肥和使用养殖水进行农田灌溉时,需要关注磺胺二甲嘧啶的生态效应,保障水稻等作物的安全生产.

Effects of Sulfamethazine in Soil on Rice Growth

For the sake of investigating the effects of residual antibiotics in soil on plant growth, sulfamethazine, which is commonly detected in soil, was selected in this project. In general, the growth index of rice at the seedling and mature stages, physiological/biochemical characteristics of roots and leaves, antibiotic residues, enrichment factors, and transport coefficients in various rice organs were respectively tested and analyzed to evaluate the ecological effects of sulfamethazine residues on rice. The results revealed that the inhibitory effect of sulfamethazine on plant height and biomass was maintained during the whole growth cycle. Moreover, the effect at the seedling stage was greater than that at the growth maturity stage, and the root part was more easily influenced than the seedling section. The root activity, nitrate reductase activity, and leaf chlorophyll content at the seedling stage were hindered by the increase in antibiotic content. By contrast, the antioxidant enzyme change showed a different tendency, in which the superoxide was activated, and the catalase and peroxidase were firstly activated and then inhibited. The sulfamethazine accumulation in various rice organs was in the order of root>leaf>sti>grain. The results of antibiotic risk assessment of rice grains exhibited that EDI/ADI was less than 0.1, indicating no health risk. The effect of sulfamethazine on enrichment factors and transport coefficients at the growth maturity stage was more obvious than that at the seedling stage. Considering the adverse effects of sulfamethazine on rice, we need to take the ecological effects of sulfamethazine on plants into consideration when applying livestock manure as organic fertilizer or using aquaculture water for irrigation, to ensure crop production safety.