快速检测毒死蜱残留量的酶膜生物传感器研究

毒死蜱是一种当前使用广泛的中等毒性有机磷农药．采用传统方法检测毒死蜱．难以满足现场检测的需要。以戊二醛为交联剂．制备了一种基于乙酰胆碱酯酶的酶膜生物传感器．并将其应用于毒死蜱的检测。考察了不同pH值．戊二醛用量．工作环境温度对传感器工作过程的影响。结果表明．本酶膜传感器可定量检出浓度为1．64～20．52mg／kg的毒死蜱。

Study on Enzyme-Membrane Biosensor for Fast Detection of Dursban Residua

Dursban is one of the widely used organophos-phorus pesticides. Conventional methods for detecting dursban are hard to satisfy the requisite of online determination. In present paper, an acetylcholinest erase-based biosensor was fabricated by using glutaraldehyde as a cross-linking agent, and used for detecting dursban residua. The effects of concentration of glutaraldehyde, different pH and biosensor's working temperature on determination of biosensor have been studied. The immobilized enzyme-membrane biosensor could make a quantitative detection of dursban in the concentration range 1.64 - 20,52mg/kg.