| 添加磷素对低磷稻田根际土壤固碳自养微生物数量的影响 |
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| 引用本文： | 柏菁,李奕霏,刘守龙,祝贞科,李科林,彭佩钦,吴小红,葛体达,吴金水. 添加磷素对低磷稻田根际土壤固碳自养微生物数量的影响[J]. 环境科学, 2018, 39(12): 5672-5679 |
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| 作者姓名： | 柏菁 李奕霏 刘守龙 祝贞科 李科林 彭佩钦 吴小红 葛体达 吴金水 |
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| 作者单位： | 中南林业科技大学环境科学与工程学院;中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室;中南林业科技大学生命科学与技术学院;江西省南昌县莲塘一中;;中国科学院亚热带农业生态...;中国科学院亚热带农业生态...;桂林理工大学岩溶地区水污...;山东建筑大学市政与环境工...;亚热带农业生态过程重点实...;中国地质大学(武汉);上...;青岛农业大学资源与环境学...;西北工业大学应用化学系;;湖南农业大学生物科学技术... |
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| 基金项目： | 国家自然科学基金项目（41503081，41771337） |
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| 摘 要： | 为了探讨磷素添加对低磷稻田土壤固碳自养微生物数量的影响,以低磷水稻土为研究对象,设置添加磷素(P添加量为80 mg·kg~(-1))和不添加磷素两种处理土壤,种植水稻进行室内培养实验.利用实时荧光定量PCR(real-time PCR)技术分析了水稻分蘖期(移栽后14 d)和拔节期(移栽后22 d)添加磷素(P)和对照(CK)处理根际土壤固碳自养微生物cbb L、cbb M、acc A和acl B基因数量的差异,同时测定了土壤理化性质,并分析了不同处理方式下固碳功能基因丰度与土壤理化性质之间的关系.结果表明,分蘖期磷素添加降低了土壤MBC和NH+4-N含量,提高了土壤DOC、Olsen-P和p H;分蘖期P处理NO-3-N含量比CK处理低,而拔节期反而比CK处理高.分蘖期,磷素添加显著提高了cbb L、cbb M、acc A和acl B基因的数量,与CK处理相比,分别提高156%、99%、110%和193%.而磷素添加对拔节期cbb L、acc A和acl B基因数量的促进作用并不明显,对cbb M基因数量反而产生了抑制作用.冗余分析(RDA)显示,土壤Olsen-P含量是影响固碳自养微生物丰度最显著的环境因子.
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| 关 键 词： | 磷素 低磷水稻土 根际土壤 固碳自养微生物 丰度 |
| 收稿时间： | 2018-03-29 |
| 修稿时间： | 2018-06-04 |
Effect of Phosphorus Addition on the Abundance of Autotrophic CO2-Fixation Microorganisms in Rhizospheric Soil from a Phosphorus-Limited Paddy Field |
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| BAI Jing,LI Yi-fei,LIU Shou-long,ZHU Zhen-ke,LI Ke-lin,PENG Pei-qin,WU Xiao-hong,GE Ti-da and WU Jin-shui. Effect of Phosphorus Addition on the Abundance of Autotrophic CO2-Fixation Microorganisms in Rhizospheric Soil from a Phosphorus-Limited Paddy Field[J]. Chinese Journal of Environmental Science, 2018, 39(12): 5672-5679 |
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| Authors: | BAI Jing LI Yi-fei LIU Shou-long ZHU Zhen-ke LI Ke-lin PENG Pei-qin WU Xiao-hong GE Ti-da WU Jin-shui |
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| Affiliation: | College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China,College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China,Key Laboratory of Agro-Ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,Key Laboratory of Agro-Ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China,College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China,College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China,College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China,Key Laboratory of Agro-Ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China and Key Laboratory of Agro-Ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China |
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| Abstract: | A rice pot experiment was conducted to investigate the effect of phosphorus addition on the abundance of autotrophic CO2-fixation microorganisms using phosphorus-limited paddy soil from the Changsha Observation and Research Station for the Agricultural Environment. Rice seedlings were transplanted in the paddy soil with or without phosphorus addition, corresponding to P-treated-pot (P) or control pot (CK), respectively. Rhizosphere soils were collected from the P and CK treatments during the tillering and shooting stages. The physical and chemical soil properties were measured and the abundance of autotrophic CO2-fixation microorganisms was quantified with a real-time PCR technique based on four functional genes (cbbL, cbbM, accA, and aclB) involved in three CO2-fixation pathways (CBB cycle, rTCA cycle, and 3-hydroxypropionate/4-hydroxybutyrate cycle). The results show that phosphorus addition improves the concentrations of DOC and Olsen-P and the pH value, whereas negative effects on the MBC and NH4+-N concentrations are revealed during the tillering stage. The effect of phosphorus addition on the NO3--N concentration in the tillering and shooting stages differs. Phosphorus addition significantly increases the abundances of the cbbL, cbbM, accA, and aclB genes, which are 156%, 99%, 110%, and 193% higher than those of the CK treatment in the tillering stage. However, this positive effect is not notable for the cbbL, accA, and aclB genes during the shooting stage. Redundancy analysis (RDA) shows that Olsen-P is the environmental factor that most significantly affects the abundance of autotrophic CO2-fixation microorganisms. |
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| Keywords: | phosphorus phosphorus-limited paddy soil rhizosphere soil autotrophic CO2-fixation microorganism gene abundance |
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