| 两株重金属抗性细菌对铅镉吸附特性的比较研究 |
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| 引用本文： | 燕传明,贺卓,葛占标,盛下放,何琳燕. 两株重金属抗性细菌对铅镉吸附特性的比较研究[J]. 环境科学学报, 2018, 38(9): 3597-3604 |
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| 作者姓名： | 燕传明 贺卓 葛占标 盛下放 何琳燕 |
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| 作者单位： | 农业部农业环境微生物重点实验室南京农业大学生命科学学院 |
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| 基金项目： | 国家自然科学基金（No.41471273） |
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| 摘 要： | 针对目前重金属水污染现状,为探究微生物法修复重金属水污染机制,本试验以抗重金属革兰氏阴性菌Rhizobium sp.W33与革兰氏阳性菌Bacillus sp.H3为供试菌株,对不同重金属(Pb、Cd)浓度及pH条件下的菌株生长状况进行测定;比较不同状态细胞(Growing、Grown、Dead)的吸附能力;利用FTIR初步分析吸附相关官能团.结果表明:两株菌对Pb抗性较强,对Cd抗性相对较弱,最适生长pH均在6~7范围内;不同状态细胞的吸附能力大小为:GrownDeadGrowing;随着重金属浓度的升高,Rhizobium sp.W33与Bacillus sp.H3的吸附容量逐渐增大,且对铅镉的吸附主要以胞外吸附为主,对铅有一定的胞内积累能力;FTIR分析显示菌株吸附过程中主要是—OH、—NH、—CO、—CH2官能团在生物吸附过程中发挥作用.Rhizobium sp.W33与Bacillus sp.H3表面官能团特性及整体吸附能力存在明显差异.
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| 关 键 词： | 重金属抗性细菌 Pb Cd 胞外吸附 胞内聚集 |
| 收稿时间： | 2018-03-13 |
| 修稿时间： | 2018-04-17 |
Comparative study on adsorption characteristics of lead and cadmium by two heavy metal resistant bacteria |
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| YAN Chuanming,HE Zhuo,GE Zhanbiao,SHENG Xiafang and HE Linyan. Comparative study on adsorption characteristics of lead and cadmium by two heavy metal resistant bacteria[J]. Acta Scientiae Circumstantiae, 2018, 38(9): 3597-3604 |
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| Authors: | YAN Chuanming HE Zhuo GE Zhanbiao SHENG Xiafang HE Linyan |
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| Affiliation: | Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 and Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | |
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| Keywords: | heavy metal resistant bacteria Pb Cd extracellular adsorption intracellular aggregation |
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