利用磷脂脂肪酸表征白洋淀沉积物微生物特征

以白洋淀表层沉积物为研究对象,运用磷脂脂肪酸(PLFA)技术分析了沉积物中微生物的生物量和群落结构.结果表明,表层沉积物中支链饱和脂肪酸含量最高,细菌总数平均值为4.24×109个/g(以干重计),是沉积物中微生物的主体.厌氧细菌和革兰氏阳性菌是细菌的主要类型,同时存在较高的好氧细菌生物量.相关分析表明,水深对沉积物细菌生物量及多样性影响显著,磷可能是影响沉积物中微生物生物量及丰富度的限制性营养因素.主成分分析表明白洋淀沉积物微生物群落结构表现出较强的区域分布特征.

Microbial characteristics in the sediments of Baiyangdian Lake utilizing phospholipid fatty acids analysis

Microbial biomass and community structure in surface sediments of Baiyangdian Lake were studied with the technology of phospholipid fatty acid(PLFA) analysis. The results showed that there were mostly branched chain saturated fatty acids in sediments and the dominated bacterial biomass averaged 4.24×109 cells/g(counted by dry weight). Anaerobic bacteria and G+ were the major microbial type, while there were still large amount of aerobic bacteria in surface sediments. Correlation analysis indicated that water depth had great effect on sediment bacterial biomass and biodiversity. Phosphorus could be the limited nutrient factor for the microbial biomass and abundance in sediments. Principle component analysis (PCA) showed that there were region distribution characteristics in sediment microbial community structure.