硝化产物对硝化细菌混培物保藏特性的影响

采用添加硝化产物的方法,研究了饥饿保藏过程中硝化产物对硝化细菌混培物活性和相关性状的影响.结果表明,饥饿保藏过程中添加亚硝酸盐可改善硝化细菌混培物的保藏效果,并明显降低硝化活性的衰减速率.缺氧条件下,4℃加5mmol/L亚硝态氮、4℃加5mmol/L硝态氮及4℃不加代谢产物3种保藏方法下的硝化活性均呈指数衰减,衰减速率分别为0.010d-1、0.020d-1、0.021d-1,半衰期分别为72.3、33.9、32.7d;硝化活性趋于稳定时,3种方法下的硝化活性保留率分别为18.7%、19.1%和15.1%,总菌体量保留率分别为62.0%、47.3%和58.7%;随着饥饿保藏时间的延长,各方法下的硝化细菌混培物ATP含量和血红素c含量均随硝化活性的衰减而下降,混培物颜色也随硝化活性的衰减而变黑.

Influence of nitration product on the preservation characteristics of mixed nitrifying culture

Influence of nitration product on the preservation characteristics of mixed nitrifying culture was studied. Nitrite could decrease the decay rate of nitrifying activity, and also improve the other characteristics during the starvation period. Under the anaerobic conditions of 4℃ with 5mmol/L nitrite, 4℃ with 5mmol/L nitrate and 4℃, the decay rate of nitrifying activity was 0.010/d, 0.020/d and 0.021/d, and the half-life was 72.3, 33.9 and 32.7d, respectively. After 5months’ preservation, the relative activity survival and relative biomass survival were 18.7%, 19.1%, 15.1%, and 62.0%, 47.3%, 58.7%, respectively. With storage time prolonging and the activity decayed, the heme c and ATP contents decreased, and the mixed nitrifying culture changed to black gradually.