利用SOS/umu测试方法鉴定沙颍河河水中的遗传毒性物质

采用HPLC分割导向的SOS/umu测试方法鉴定了沙颍河河水中的遗传毒性物质。当采用TA1535/pSK1002菌株测定HPLC分割的各馏分时,如果不经大鼠肝微粒体酶(S9)代谢活化,只有馏分F10显示遗传毒性,加入大鼠肝微粒体酶代谢活化后,馏分F10和馏分F15都显示有遗传毒性,说明河水中存在某些需经过大鼠肝微粒体酶代谢活化才能显示出遗传毒性的物质。当采用过量表达O-乙酰转移酶(O-AT),对芳香胺类物质和硝基芳烃化合物有特殊响应的NM2009菌株测定时,馏分F8、F9和F10均呈现遗传毒性;特别是对于馏分F10,用NM2009菌株测定的遗传毒性远高于原始菌,说明这3个馏分中都含有芳香胺类或硝基芳烃类物质。

SOS/umu Test for Detection of Genotoxins in Water of Shaying River

In the present study, HPLC fractionation directed SOS/umu assay was performed to identify the genotoxic chemicals in Shaying River water samples. After HPLC fractionation, every fraction was tested. Genotoxic activity was observed only in Fraction 10 upon using TA1535/pSK1002 strain without rat liver microsomes (S9) addition, while genotoxic activities were observed in both Fraction 10 and 15 by using same strain with rat liver microsomes addition, indicating that some genotoxins in water samples elicited their genotoxicity under rat liver microsomes metabolic activation. NM2009 strain can over-express O-AT and show specific response to aromatic amines and nitro-aromatic compounds. When NM2009 strain was used to test, the genotoxic activities were detected in Fractions 8, 9 and 10. Especially, the genotoxic activity of Fraction 10 was greater than that when using TA1535/pSK1002 strain to test. These results indicated that the major causative agents of Fraction 8, 9, and 10 should be aromatic amines or nitroarenes.