纤维素酶气相双动态固态发酵

为了充分利用纤维素酶固态发酵的优势,提出了纤维素酶气相双动态固态发酵的方式.研究结果表明:在优化条件下(最佳压力脉冲范围、脉冲频率及气体内循环速率),发酵温度得到较好地控制,9.0cm高的填料层中最大温度梯度为0.12℃/cm;以汽爆秸秆为底物,发酵水活度得到较好的保持;动态培养发酵周期(60h)比静态发酵周期(84h)缩短了 1/3,酶活(20.36IU/g)比静态酶活(10.82IU/g)提高了1倍,压力脉动固态培养的料层上中下微生物生长状况均匀一致,且疏松,而静态固态发酵的料层中部几乎没有菌体生长利用气相双动态固态发酵可为纤维素酶大规模生产奠定基础.

The Solid-state Fermentation with Double Dynamic of Gas Phase for Celluloses

To utilize the advantages of solid-state fermentation for cellulose production, the solid-state fermentation with double dynamic of gas phase was proposed. The studies showed as follows: under the optimum operating conditions, the air pulsation range, the air pulsation periods and the air internal circulation, the fermentation temperatures were controlled quite well and the maximum temperature difference was 0.12 degree C/cm at a bed height of 9.0 cm; the water activity in the fermentation with steam-exploded straw as substrate was very suitable for the fungal growth; and with the observation of microbial growth by ESEM. It was discovered that air-pulsating fermentation was more advantageous to cellulose production and microorganism growth than static fermentation. The period of air-pulsating fermentation (60 h) was almost shorter 1/3 and its filter paper activity of enzymes (20.36 IU/mg) was larger 1 time than in the static fermentation with the fermentation period 84 h and the enzyme activity (10.82 IU/mg). Thus, this double dynamic of gas laid strong basis for cellulose production on a large scale.